لَا تَكْتُبْ
Do not write.
This is prohibition: prohibitive lā makes the verb jussive and asks someone to refrain.
لَا تَكْتُبُ
You do not write.
This is a negative statement: negative lā does not put the verb in the jussive, so “taktubu” remains indicative.
Both inflection and communicative purpose matter. A prohibition requests abstention, while negation reports the absence of an action or state.