The same particle can appear in two different uses. Look at the verb ending to tell them apart.
MeaningFormUse
he does not helpلَا يَنْصُرُNegation; no Jazm
let him not helpلَا يَنْصُرْProhibition; Jazm
you do not helpلَا تَنْصُرُوْنَNegation; Noon remains
do not helpلَا تَنْصُرُوْاProhibition; Noon drops